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Western blot analysis in 293 cell line lysates (35ug/lane).
ECT2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung squamous carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in 293 cell line lysates (35ug/lane).

ECT2 Antibody

CATALOG NUMBER: 57-964

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This ECT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 597-625 amino acids from the Central region of human ECT2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
104 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ECT2
Additional Names:
Protein ECT2, Epithelial cell-transforming sequence 2 oncogene, ECT2
Protein Accession Number:
Q9H8V3
Protein Gi Number:
357529579
Ncbi Gene Id Number:
1894
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a transforming proteinthat is related to Rho-specific exchange factors and yeast cellcycle regulators. The expression of this gene is elevated with theonset of DNA synthesis and remains elevated during G2 and M phases.In situ hybridization analysis showed that expression is at a highlevel in cells undergoing mitosis in regenerating liver. Thus, thisprotein is expressed in a cell cycle-dependent manner during liverregeneration, and is thought to have an important role in theregulation of cytokinesis.
Background Reference 1:
Justilien, V., et al. Oncogene 28(41):3597-3607(2009)
Background Reference 2:
Burkard, M.E., et al. PLoS Biol.(5), E1000111 (2009) :
Background Reference 3:
Wolfe, B.A., et al. PLoS Biol.(5), E1000110 (2009) :
Background Reference 4:
Hirata, D., et al. Clin. Cancer Res. 15(1):256-266(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-964

List Size:
400 ul

List Price:

$495.00

Datasheet
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