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Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.

EDA Antibody

CATALOG NUMBER: 25-972

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EDA.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
EDA antibody can be used for detection of EDA by ELISA at 1:312500. EDA antibody can be used for detection of EDA by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
41 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store EDA antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
EDA
Additional Names:
EDA, ED1, ED1-A1, ED1-A2, EDA1, EDA2, HED, XHED, XLHED, HED1, ODT1, ECTD1, EDA-A1, EDA-A2, STHAGX1
Protein Accession Number:
NP_001005609
Protein Gi Number:
54112101
Ncbi Gene Id Number:
1896
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
EDA is a type II membrane protein that can be cleaved by furin to produce a secreted form. It belongs to the tumor necrosis factor family, acts as a homotrimer and may be involved in cell-cell signaling during the development of ectodermal organs. Defects in this gene are a cause of ectodermal dysplasia, anhidrotic, which is also known as X-linked hypohidrotic ectodermal dysplasia. Several transcript variants encoding many different isoforms have been found for this gene. The protein encoded by this gene is a type II membrane protein that can be cleaved by furin to produce a secreted form. The encoded protein, which belongs to the tumor necrosis factor family, acts as a homotrimer and may be involved in cell-cell signaling during the development of ectodermal organs. Defects in this gene are a cause of ectodermal dysplasia, anhidrotic, which is also known as X-linked hypohidrotic ectodermal dysplasia. Several transcript variants encoding many different isoforms have been found for this gene.
Background Reference 1:
Zhao, J., (2008) Br. J. Dermatol. 158 (3), 614-617.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

25-972

List Size:
100 ul

List Price:

$519.00

Datasheet
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