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Western blot analysis of EDA1 in mouse kidney tissue lysate with EDA1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of EDA1 in mouse kidney tissue with EDA1 antibody at 5 μg/ml.
Immunofluorescence of EDA1 in mouse kidney tissue with EDA1 antibody at 20 μg/ml.
Western blot analysis of EDA1 in mouse kidney tissue lysate with EDA1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

EDA1 Antibody

CATALOG NUMBER: 8045

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (89%)
Immunogen:
EDA1 antibody was raised against an 19 amino acid peptide near the center of human EDA1.
The immunogen is located within amino acids 130 - 180 of EDA1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
EDA1 antibody can be used for detection of EDA1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
EDA1 antibody is human, mouse and rat reactive. Multiple isoforms of EDA1 are known to exist.
Positive Control 1:
Cat. No. 1405 - Mouse Kidney Tissue Lysate
Predicted Molecular Weight:
Predicted: 16, 43 kDa
Observed: 39 kDa
Purification:
EDA1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
EDA1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
EDA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
EDA
Additional Names:
Ectodysplasin A, Ectodermal dysplasia protein, ED1, ED1-A1, ED1-A2, EDA1, EDA2, EDA protein, HED, ODT1, STHAGX1, XHED, XLHED
Protein Accession Number:
NP_001390
Protein Gi Number:
4503449
Ncbi Gene Id Number:
1896
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Ectodysplasin A (EDA1) is a member of the TNF-related ligand family involved in the early epithelial-mesenchymal interaction that regulates ectodermal appendage formation (1). It is a trimeric type II membrane protein that co-localizes with cytoskeletal structures at the lateral and apical surfaces of cells and can be expressed as eight alternatively spliced isoforms in hair follicles and in the epidermis of adult skin (2,3). EDAs are required during development, and loss or mutation of EDA1 results in a group of developmental disorders identified as ectodermal dysplasia type 1 (4,5).
Background Reference 1:
Kere J, Srivastava AK, Montonen O. X-linked anhidrotic (hypohidrotic) ectodermal dysplasia is caused by mutation in a novel transmembrane protein. Nat. Genet. 1996; 13:409-16.
Background Reference 2:
Vincent MC, Biancalana V, Ginisty D, et al. Mutational spectrum of the ED1 gene in X-linked hypohidrotic ectodermal dysplasia. Eur. J. Hum. Genet. 2001; 9:355-63.
Background Reference 3:
Ohashi M, Moriya C, Tanahashi K, et al. A new EDA gene mutation in a family of X-linked hypohidrotic ectodermal dysplasia. J. Dermatol. Sci. 2014; 74:175-7.
Background Reference 4:
Bayés M, Hartung AJ, Ezer S, et al. The anhidrotic ectodermal dysplasia gene (EDA) undergoes alternative splicing and encodes ectodysplasin-A with deletion mutations in collagenous repeats. Hum. Mol. Genet. 1998; 7:1661-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8045

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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