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43-408 (0.3ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-408 (5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-408 (0.3ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

EDF1 Antibody

CATALOG NUMBER: 43-408

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig
Immunogen:
The immunogen for this antibody is: C-NKQHSITKNTAKLDR
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 17kDa band observed in lysates of cell line A549 (calculated MW of 16.4kDa according to NP_003783.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Spleen shows nuclear staining of select lymphocytes in the marginal zone. Recommended concentration, 5-10ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_003783.1; NP_694880.1).
Predicted Molecular Weight:
Approx 17kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
EDF1
Additional Names:
EDF1, endothelial differentiation-related factor 1, EDF-1, MBF1, multiprotein bridging factor 1
Protein Accession Number:
NP_003783.1,NP_694880.1
Protein Gi Number:
4503453
Ncbi Gene Id Number:
8721
Background Reference 1:
Brendel C, Gelman L, Auwerx J.Multiprotein bridging factor-1 (MBF-1) is a cofactor for nuclear receptors that regulate lipid metabolism.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-408

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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