Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

43-596 (0.3ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-596 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle/cytoplasmic staining. The nuclear stain i
43-596 (0.3ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

EEA1 Antibody

CATALOG NUMBER: 43-596

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Immunogen:
The immunogen for this antibody is: C-QETKIQHEELNNRIQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 200kDa band observed in lysates of cell line HEK293 (calculated MW of 162kDa according to NP_003557.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml. Primary incubatiion 1 hour at room temperature. Immunofluorescence :Strong expression of the protein seen in the vesicles/cytoplasm of NIH3T3 cells. Recommended concentration: 5ug/ml.
Predicted Molecular Weight:
Approx 200kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
EEA1
Additional Names:
EEA1, early endosome antigen 1, MST105, MSTP105, ZFYVE2, early endosome antigen 1, 162kD, early endosome-associated protein, endosome-associated protein p162, zinc finger FYVE domain-containing protein 2
Protein Accession Number:
NP_003557.2
Protein Gi Number:
55770888
Ncbi Gene Id Number:
8411
Background Reference 1:
Mishra A, Eathiraj S, Corvera S, Lambright DG.Structural basis for Rab GTPase recognition and endosome tethering by the C2H2 zinc finger of Early Endosomal Autoantigen 1 (EEA1).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-596

List Size:
0.1 mg

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369