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Antibody used in WB on Human HepG3 at 1 ug/ml.
Antibody used in WB on HepG2 at 4.0 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Antibody used in WB on Human HepG3 at 1 ug/ml.

EED Antibody

CATALOG NUMBER: 28-854

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EED.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
EED antibody can be used for detection of EED by ELISA at 1:312500. EED antibody can be used for detection of EED by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
46 kDa, 50 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store EED antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
EED
Additional Names:
EED, HEED, WAIT1
Protein Accession Number:
NP_694536
Protein Gi Number:
24041023
Ncbi Gene Id Number:
8726
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function.This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene.
Background Reference 1:
Witte, V., (2004) Mol. Cell 13 (2), 179-190.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-854

List Size:
100 ul

List Price:

$519.00

Datasheet
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