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Western blot analysis of EED in human heart tissue lysate with EED antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of EED in human heart tissue with EED antibody at 20 μg/mL.
Western blot analysis of EED expression in human testis tissue lysate with EED antibody at 2 μg/ml.
Western blot analysis of EED in human heart tissue lysate with EED antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

EED Antibody

CATALOG NUMBER: 7009

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (100%), Bovine: (100%)
Immunogen:
EED antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human EED.
The immunogen is located within amino acids 30 - 80 of EED.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
EED antibody can be used for detection of EED by Western blot at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
Four alternatively spliced transcript variants have been observed.
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Positive Control 2:
Cat. No. 10-501 - Human Heart Tissue Slide
Predicted Molecular Weight:
Predicted: 49 kDa
Observed: 49 kDa
Purification:
EED Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
EED Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
EED antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EED
Additional Names:
EED Antibody: HEED, WAIT1, Polycomb protein EED, WD protein associating with integrin cytoplasmic tails 1, hEED
Protein Accession Number:
NP_003788
Protein Gi Number:
24041020
Ncbi Gene Id Number:
8726
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
EED Antibody: The EED protein (WAIT-1 or WD protein associated with integrin cytoplasmic tails-1), also called embryonic ectoderm development, is a member of the superfamily of WD-40 repeat proteins and widely conserved polycomb group (PcG) family of proteins. The polycomb group (PcG) is a large and evolutionarily conserved set of genes whose products act in multimeric complexes to modify histones, which are then thought to cause stable and heritable states of transcriptional repression. EED shuttles between the nucleus and the plasma membrane and can interact with the cytoplasmic tail of integrin β7 subunit. EED exerted an antiviral activity at the late stage of HIV-1 replication.
Background Reference 1:
Rietzler M, Bittner M, Kolanus W, et al. The human WD repeat protein WAIT-1 specifically interacts with the cytoplasmic tails of beta7-integrins. J. Biol. Chem. 1998; 273:27459-66.
Background Reference 2:
Brock HW and Fisher CL. Maintenance of gene expression patterns. Dev Dyn. 2005; 232:633-55.
Background Reference 3:
Levine SS, King IF, Kingston RE, et al. Division of labor in polycomb group repression. Trends Biochem. Sci. 2004; 29: 478-85.
Background Reference 4:
Van der Vlag J and Otte AP. Transcriptional repression mediated by the human Polycomb-group protein EED involves histone deacetylation. Nat. Genet.1999; 23:474-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7009

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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