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43-205 (0.5ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-205 (0.5ug/ml) staining of Mouse (A+C) and Rat (B+D) Skeletal Muscle (A+B) and Heart (C+D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-205 (0.5ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

EEF1A2 Antibody

CATALOG NUMBER: 43-205

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-NPATVPFVPIS
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 50kDa band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 50.5kDa according to NP_001949.1). The same band is observed in lysates of Mouse and Rat Heart and Skeletal Muscle. Recommended concentration: 0.5-2ug/ml.
Predicted Molecular Weight:
Approx 50kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
EEF1A2
Additional Names:
EEF1A2, eukaryotic translation elongation factor 1 alpha 2, EEF1AL, EF-1-alpha-2, EF1A, FLJ41696, HS1, STN, STNL, OTTHUMP00000031776, eEF1A-2, elongation factor 1-alpha 2, elongation factor-1 alpha, eukaryotic elongation factor 1 A-2, statin S1, statin-S1
Protein Accession Number:
NP_001949.1
Protein Gi Number:
4503475
Ncbi Gene Id Number:
1917
Background Reference 1:
Li Z, Qi CF, Shin DM, Zingone A, Newbery HJ, Kovalchuk AL, Abbott CM, Morse HC 3rd.Eef1a2 promotes cell growth, inhibits apoptosis and activates JAK/STAT and AKT signaling in mouse plasmacytomas.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-205

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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