Western blot analysis of extracts of various cell lines, using EEF1B2 antibody (22-328) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

Western blot analysis of extracts from normal (control) and EEF1B2 knockout (KO) 293T cells, using EEF1B2 antibody (22-328) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat spinal cord using EEF1B2 Antibody (22-328) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using EEF1B2 Antibody (22-328) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using EEF1B2 Antibody (22-328) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A549 cells using EEF1B2 antibody (22-328). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using EEF1B2 antibody (22-328) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

EEF1B2 Antibody, KO Validated

CATALOG NUMBER: 22-328

Clonality:

Polyclonal

Tested Applications:

IF, IHC, WB

Host Species:

Rabbit

Species Reactivity:

Human, Mouse, Rat

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human, Mouse, Rat

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human EEF1B2 (NP_001032752.1).

Conjugate:

Unconjugated

Tested Applications:

IF, IHC, WB

Application Note:

WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200

Positive Control 1:

MCF-7

Positive Control 2:

HL-60

Positive Control 3:

HeLa

Positive Control 4:

293T

Positive Control 5:

Mouse pancreas

Predicted Molecular Weight:

Observed: 33kDa

Advanced Validation

Validation:

Antibody is Knockout validated.

Properties

Purification:

Affinity purification

Clonality:

Polyclonal

Isotype:

IgG

Physical State:

Liquid

Buffer:

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration:

batch dependent

Storage Conditions:

Store at -20°C. Avoid freeze / thaw cycles.

Additional Info

Ncbi Official Symbol:

EEF1B2

Additional Names:

Elongation factor 1-beta, EF-1-beta, EEF1B2, EEF1B, EF1B

Ncbi Gene Id Number:

1933

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

This gene encodes a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. Alternative splicing results in three transcript variants which differ only in the 5' UTR.

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-328

List Size:

100 uL

List Price:

$540.00

Datasheet

Shipping Info

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EEF1B2 Antibody, KO Validated

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Positive Control 1:

Positive Control 2:

Positive Control 3:

Positive Control 4:

Positive Control 5:

Predicted Molecular Weight:

Validation:

Purification:

Clonality:

Isotype:

Physical State:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Ncbi Gene Id Number:

User Note:

Background:

Disclaimer:

List Size:

Shipping Info

Customize your order

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