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Western blot analysis of extracts of HepG2 cells, using EEF1E1 antibody (19-338).<br/>Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts of HepG2 cells, using EEF1E1 antibody (19-338).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

EEF1E1 Antibody [32A7]

CATALOG NUMBER: 19-338

Clonality:
Monoclonal
Tested Applications:
Flow, IF, WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Immunogen:
A synthetic peptide of human EEF1E1
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, WB
Application Note:
WB: 1:500 - 1:1000
IF: 1:20 - 1:50
Flow: 1:20 - 1:50
Positive Control 1:
HepG2
Predicted Molecular Weight:
Observed: 20kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
EEF1E1
Additional Names:
EEF1E1, eukaryotic translation elongation factor 1 epsilon 1, AIMP3, P18, ARS-interacting multifunctional protein 3, aminoacyl tRNA synthetase complex-interacting multifunctional protein 3, eukaryotic translation elongation factor 1 epsilon-1
Ncbi Gene Id Number:
9521
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage, and it plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream MUTED (muted homolog) gene. An EEF1E1-related pseudogene has been identified on chromosome 2.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-338

List Size:
100 uL

List Price:

$365.00

Out of stock

Datasheet
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