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Western blot analysis of EFCAB4B in mouse kidney tissue lysate with EFCAB4B antibody at 1 μg/mL.
Immunohistochemistry of EFCAB4B in human kidney tissue with EFCAB4B antibody at 10 μg/mL.
Immunofluorescence of EFCAB4B in human kidney tissue with EFCAB4B antibody at 20 μg/mL.
Western blot analysis of EFCAB4B in mouse kidney tissue lysate with EFCAB4B antibody at 1 μg/mL.
Trial Size

EFCAB4B Antibody

CATALOG NUMBER: 6045

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
EFCAB4B antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human EFCAB4B.
The immunogen is located within amino acids 640 - 690 of EFCAB4B.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
EFCAB4B antibody can be used for detection of EFCAB4B by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1405 - Mouse Kidney Tissue Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Purification:
EFCAB4B Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
EFCAB4B Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
EFCAB4B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EFCAB4B
Additional Names:
EFCAB4B Antibody: CRACR2A, CRACR2A, EF-hand calcium-binding domain-containing protein 4B, Calcium release-activated calcium channel regulator 2A, CRAC channel regulator 2A
Protein Accession Number:
NP_001138430
Protein Gi Number:
14249254
Ncbi Gene Id Number:
84766
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
EFCAB4B Antibody: EFCAB4B, also known as Calcium release-activated calcium channel regulator 2A, is a novel Ca2+-binding EF-hand protein that is thought to play a key role in store-operated Ca2+ entry in T-cells by regulating CRAC channel activation. EFCAB4B acts as a cytoplasmic calcium-sensor that forms a complex with ORAI1 and STIM1 at the junctional regions between the plasma membrane and the endoplasmic reticulum upon low Ca2+ concentration. A closely related protein, EFCAB4A, is likely to play a similar role as EFCAB4B, but the detailed function of EFCAB4A is still under investigation.
Background Reference 1:
Srikanth S, Jung HJ, Kim KD, et al. A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells. Nat. Cell. Biol.2010; 12:436-46.
Background Reference 2:
Srikanth S, Jung HJ, Ribalet B, et al. The intracellular loop of Orai1 plays a central role in fast inactivation of Ca2+ release-activated Ca2+ channels. J. Biol. Chem.2010; 285:5066-75.
Background Reference 3:
Maruyama K, Mikawa T, and Ebashi S. Detection of calcium binding proteins by 45Ca autoradiography on nitrocellulose membrane after sodium dodecyl sulfate gel electrophoresis. J. Biochem.1984; 95:511-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6045

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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