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Western blot analysis of EFHD1 in human spleen tissue lysate with EFHD1 antibody at (A) 2 and (B) 4 μg/mL.
Immunofluorescence of EFHD1 in rat spleen tissue with EFHD1 antibody at 5 μg/mL.
Western blot analysis of EFHD1 in human spleen tissue lysate with EFHD1 antibody at (A) 2 and (B) 4 μg/mL.
Trial Size

EFHD1 Antibody

CATALOG NUMBER: 5655

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
EFHD1 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human EFHD1.
The immunogen is located within the first 50 amino acids of EFHD1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
EFHD1 antibody can be used for detection of EFHD1 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunoflourescence starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Purification:
EFHD1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
EFHD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
EFHD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EFHD1
Additional Names:
EFHD1 Antibody: SWS2, MST133, PP3051, MSTP133, SWS2, EF-hand domain-containing protein D1, EF-hand domain-containing protein 1
Protein Accession Number:
NP_079478
Protein Gi Number:
20149496
Ncbi Gene Id Number:
80303
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
EFHD1 Antibody: EFHD1, also known as Swiprosin-2 or SWS2, is an EF-hand and coiled-coil-containing adaptor protein identified in a subtractive hybridization study using a neuronal cell line established from the cerebellum of an adult p53-null mouse, however further study indicated no difference in normal mice. Its mRNA is widely expressed, with its expression in brain undetectable at embryonic stages, with increasing levels from postnatal to adult development. In situ hybridization showed expression in neurons but not white matter of the cerebellum and cerebrum. EHFD1 is also highly expressed in testes, ovary, and the collecting ducts of the kidney, suggesting that in non-neuronal cells, EFHD1 may be involved in gametogenesis and water-reabsorbtion.
Background Reference 1:
Tominaga M and Tomooka Y. Novel genes cloned from a neuronal cell line newly established from a cerebellum of an adult p53-/- mouse. Biochem. Biophys. Res. Comm.2002; 297:473-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5655

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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