Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of EFHD2 in mouse brain tissue lysate with EFHD2 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of EFHD2 in human brain tissue with EFHD2 antibody at 5 μg/mL.
Immunofluorescence of EFHD2 in human brain tissue with EFHD2 antibody at 20 μg/mL.
Western blot analysis of EFHD2 in mouse brain tissue lysate with EFHD2 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

EFHD2 Antibody

CATALOG NUMBER: 5657

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
EFHD2 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human EFHD2.
The immunogen is located within the first 50 amino acids of EFHD2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
EFHD2 antibody can be used for detection of EFHD2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
EFHD2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
EFHD2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
EFHD2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EFHD2
Additional Names:
EFHD2 Antibody: SWS1, SWS1, EF-hand domain-containing protein D2, Swiprosin-1
Protein Accession Number:
NP_077305
Protein Gi Number:
20149675
Ncbi Gene Id Number:
79180
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
EFHD2 Antibody: EFHD2, also known as Swiprosin-1 or SWS1, is an EF-hand and coiled-coil-containing adaptor protein that plays a role in lymphocyte physiology. EFHD2 exhibits the highest expression in CD8+ T cells and immature B cells. It provides a membrane scaffold that is required for the Syk-, SLP-65-, and PLCgamma2-dependent B-cell receptor (BCR)-induced calcium flux. EFHD2 may also regulate BCR-induced immature and primary B-cell apoptosis. It controls spontaneous apoptosis through the regulation of BCL2L1 abundance. Also, EFHD2 plays a role as negative regulator of the canonical NF-κB-activating branch.
Background Reference 1:
Kroczek C, Lang C, Brachs S, et al. Swiprosin-1/EFhd2 controls B cell receptor signaling through the assembly of the B cell receptor, Syk, and phospholipase C gamma2 in membrane rafts. J. Immunol.2010; 184:3665-76.
Background Reference 2:
Avramidou A, Kroczek C, Lang C, et al. The novel adaptor protein Swiprosin-1 enhances BCR signals and contributes to BCR-induced apoptosis. Cell Death Differ.2007; 14:1936-47.
Background Reference 3:
Thylur RP, Kiim YD, Kwon MS, et al. Swiprosin-1 is expressed in mast cells and up-regulated through the protein kinase C beta I/eta pathway. J. Cell Biochem.2009; 108:705-15.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5657

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369