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Western blot analysis of extracts of various cell lines, using EFNA1 antibody (19-601) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human prostate using EFNA1 Antibody (19-601) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using EFNA1 Antibody (19-601) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using EFNA1 Antibody (19-601) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using EFNA1 antibody (19-601) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

EFNA1 Antibody

CATALOG NUMBER: 19-601

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 19-182 of human EFNA1 (NP_004419.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
H460
Positive Control 2:
Mouse testis
Predicted Molecular Weight:
Observed: 21-23kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
EFNA1
Additional Names:
EFNA1, tumor necrosis factor, alpha-induced protein 4, TNFAIP4, ligand of eph-related kinase 1, LERK1, immediate early response protein B61, EPLG1, ephrin-A1, ephrin A1, eph-related receptor tyrosine kinase ligand 1, EFL1, ECKLG, B61
Ncbi Gene Id Number:
1942
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNA class ephrin which binds to the EPHA2, EPHA4, EPHA5, EPHA6, and EPHA7 receptors. Two transcript variants that encode different isoforms were identified through sequence analysis.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-601

List Size:
100 uL

List Price:

$436.00

Datasheet
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