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Western blot analysis of extracts of mouse lung, using EFNB2 antibody (19-783) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat brain using EFNB2 antibody (19-783) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EFNB2 antibody (19-783) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using EFNB2 antibody (19-783). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of mouse lung, using EFNB2 antibody (19-783) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

EFNB2 Antibody

CATALOG NUMBER: 19-783

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 28-229 of human EFNB2 (NP_004084.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:10 - 1:100
Positive Control 1:
Mouse lung
Predicted Molecular Weight:
Observed: 50kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
EFNB2
Additional Names:
EFNB2, MGC126228, MGC126227, MGC126226, ligand of eph-related kinase 5, LERK5, Htk-L, HTKL, HTK ligand, EPLG5, ephrin-B2, ephrin B2, eph-related receptor tyrosine kinase ligand 5
Ncbi Gene Id Number:
1948
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-783

List Size:
100 uL

List Price:

$436.00

Out of stock

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