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EFNB2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in Hela,293 cell line lysates (35ug/lane).
Confocal immunofluorescent analysis of EFNB2 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
EFNB2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

EFNB2 Antibody

CATALOG NUMBER: 55-964

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This EFNB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 157-186 amino acids from the Central region of human EFNB2.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
37 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EFNB2
Additional Names:
Ephrin-B2, EPH-related receptor tyrosine kinase ligand 5, LERK-5, HTK ligand, HTK-L, EFNB2, EPLG5, HTKL, LERK5
Protein Accession Number:
P52799
Protein Gi Number:
1706673
Ncbi Gene Id Number:
1948
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the ephrin (EPH) family. Theephrins and EPH-related receptors comprise the largest subfamily ofreceptor protein-tyrosine kinases and have been implicated inmediating developmental events, especially in the nervous systemand in erythropoiesis. Based on their structures and sequencerelationships, ephrins are divided into the ephrin-A (EFNA) class,which are anchored to the membrane by aglycosylphosphatidylinositol linkage, and the ephrin-B (EFNB)class, which are transmembrane proteins. This gene encodes an EFNBclass ephrin which binds to the EPHB4 and EPHA3 receptors.
Background Reference 1:
Zhang, R., et al. Psychiatry Res 180(1):5-9(2010)
Background Reference 2:
Bochenek, M.L., et al. J. Cell. Sci. 123 (PT 8), 1235-1246 (2010) :
Background Reference 3:
Nakada, M., et al. Int. J. Cancer 126(5):1155-1165(2010)
Background Reference 4:
Qin, H., et al. J. Biol. Chem. 285(1):644-654(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-964

List Size:
400 ul

List Price:

$495.00

Datasheet
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