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Antibody staining EGF in Human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Antibody staining EGF in Human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution Lane 1: M.stomach tissue lysates Lane 2: A549 whole cell lysates Lysates/proteins at 20 ug per lane.
Western blot analysis in mouse stomach tissue lysates (35ug/lane).
EGF Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Antibody staining EGF in Human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

EGF Antibody

CATALOG NUMBER: 56-711

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This EGF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 690-720 amino acids from the Central region of human EGF.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:25
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
134 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EGF
Additional Names:
Pro-epidermal growth factor, EGF, Epidermal growth factor, Urogastrone, EGF
Protein Accession Number:
P01133
Protein Gi Number:
251757262
Ncbi Gene Id Number:
1950
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.
Background Reference 1:
de Diesbach, M.T., et al. Exp. Cell Res. 316(19):3239-3253(2010) Xu, Z., et al. Biochem. Biophys. Res. Commun. 401(3):376-381(2010) Lupien, M., et al. Genes Dev. 24(19):2219-2227(2010) Hommel, U., et al. J. Mol. Biol. 227(1):271-282(1992) Hernandez-Sotomayor, S.M., et al. J. Membr. Biol. 128(2):81-89(1992)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-711

List Size:
400 ul

List Price:

$495.00

Datasheet
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