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Western blot analysis in K562 cell line lysates (35ug/lane).
Western blot analysis in K562 cell line lysates (35ug/lane).

EGF Antibody

CATALOG NUMBER: 60-331

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This EGF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 940-967 amino acids from the C-terminal region of human EGF.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
134 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EGF
Additional Names:
Pro-epidermal growth factor, EGF, Epidermal growth factor, Urogastrone, EGF
Protein Accession Number:
P01133
Protein Gi Number:
251757262
Ncbi Gene Id Number:
1950
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the epidermal growth factorsuperfamily. The encoded protein is synthesized as a largeprecursor molecule that is proteolytically cleaved to generate the53-amino acid epidermal growth factor peptide. This protein acts apotent mitogenic factor that plays an important role in the growth,proliferation and differentiation of numerous cell types. Thisprotein acts by binding the high affinity cell surface receptor,epidermal growth factor receptor. Defects in this gene are thecause of hypomagnesemia type 4. Dysregulation of this gene has beenassociated with the growth and progression of certain cancers.Alternate splicing results in multiple transcript variants.
Background Reference 1:
de Diesbach, M.T., et al. Exp. Cell Res. 316(19):3239-3253(2010)
Background Reference 2:
Xu, Z., et al. Biochem. Biophys. Res. Commun. 401(3):376-381(2010)
Background Reference 3:
Lupien, M., et al. Genes Dev. 24(19):2219-2227(2010)
Background Reference 4:
Hommel, U., et al. J. Mol. Biol. 227(1):271-282(1992)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-331

List Size:
400 ul

List Price:

$495.00

Datasheet
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