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Human EGF R, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human EGF, Tag Free at 10 ug/mL (100 uL/well) can bind Human EGF R, Fc Tag with a linear range of 0.078-2.5 ug/mL (QC tested).
Immobilized Human EGF R, Fc Tag at 1 ug/mL (100 uL/well) can bind Cetuximab with a linear range of 0.1-2 ng/mL (Routinely tested).
Human EGF R, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

EGF R Recombinant Protein

CATALOG NUMBER: 11-547

Tested Applications:
ELISA, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Leu 25 - Ser 645
Fusion Tag:
Fc Tag
Tested Applications:
ELISA, WB
Application Note:
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 94.7 kDa. The protein migrates as 110-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Predicted Molecular Weight:
94.7 kDa
Purity:
>95% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
PBS, pH7.4
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
EGFR
Additional Names:
EGFR,ERBB,ERBB1,HER1,PIG61,mENA
Protein Accession Number:
NP_005219
Ncbi Gene Id Number:
1956
Background:
The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.
Background Reference 1:
Herbst RS., 2004, Int. J. Radiat. Oncol. Biol. Phys. 59 (2 Suppl): 21–6.
Background Reference 2:
Giaccone, G., 2005, Ann. Onc. 16: 538-548.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

11-547

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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