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To detect Human EGF Receptor by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human EGF Receptor (38-264) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human EGF Receptor.
To detect Human EGF Receptor by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human EGF Receptor is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Human EGF Receptor by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human EGF Receptor is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Human EGF Receptor by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human EGF Receptor (38-264) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human EGF Receptor.

EGF Receptor Antibody (biotin)

CATALOG NUMBER: 38-275

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Biotin
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits immunized with highly pure recombinant Human EGF Receptor. Human EGF Receptor specific antibody was purified by affinity chromatography and then biotinylated.
Conjugate:
Biotin
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Sandwich:
To detect Human EGF Receptor by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human EGF Receptor (38-264) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human EGF Receptor.
Western Blot:
To detect Human EGF Receptor by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human EGF Receptor is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
EGFR
Additional Names:
ERBB, HER1, mENA, ERBB1, PIG61, ERBB, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1,
Protein Accession Number:
P00533
Protein Gi Number:
2811086
Ncbi Gene Id Number:
1956
User Note:
Centrifuge vial prior to opening.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-275

List Size:
0.05 mg

List Price:

$408.00

Datasheet
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