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Western blot analysis of lysed extracts from HUVEC and MDA cells using EGFR (Ab-1070).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR (Ab-1070).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed MCF cells using EGFR (Ab-1070).
Western blot analysis of lysed extracts from HUVEC and MDA cells using EGFR (Ab-1070).

EGFR (Ab 1070) Antibody

CATALOG NUMBER: 79-310

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
EGFR (Ab-1070) antibody was raised against a peptide sequence around aa.1068~1072 (R-Y-S-S-D) derived from Human EGFR.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of total EGFR protein.
Predicted Molecular Weight:
175 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
EGFR
Additional Names:
ERBB, HER1, mENA, ERBB1, PIG61, GLUH1, GLUR1, Receptor tyrosine-protein kinase ErbB-1
Protein Accession Number:
NP_005219.2
Protein Gi Number:
29725609
Ncbi Gene Id Number:
1956
Background:
Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentia
Background Reference 1:
Feinmesser RL, et al. (1999) J Biol Chem; 274 (23): 16168-73. Gamou S, et al. (1995) FEBS Lett; 357 (2): 161-4. Gamou S, et al. (1994) J Cell Physiol; 158 (1): 151-9. Heisermann GJ, et al. (1988) J Biol Chem; 263 (26): 13152-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-310

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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