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Western blot analysis of anti-EGFR Antibody (S1070) in kidney heart tissue lysates (35ug/lane)
Western blot analysis of EGFR in Hela cell lysates, either induced (Lane 1) or noninduced with EGF (Lane 2).
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with EGFR Antibody (S1070), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-EGFR Antibody (S1070) in kidney heart tissue lysates (35ug/lane)

EGFR Antibody

CATALOG NUMBER: 63-069

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This EGFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1048-1077 amino acids from human EGFR.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
134 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EGFR
Additional Names:
Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1
Protein Accession Number:
P00533
Protein Gi Number:
2811086
Ncbi Gene Id Number:
1956
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The epidermal growth factor receptor is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.
Background Reference 1:
Zanardi, T.A., et al., J. Virol. 77(21):11685-11696 (2003).
Background Reference 2:
Krug, A.W., et al., J. Biol. Chem. 278(44):43060-43066 (2003).
Background Reference 3:
Huang, F., et al., J. Biol. Chem. 278(44):43411-43417 (2003).
Background Reference 4:
He, Y.Y., et al., J. Biol. Chem. 278(43):42457-42465 (2003).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-069

List Size:
400 ul

List Price:

$495.00

Datasheet
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