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Immunofluorescent testing of A431 cells and Alexa Fluor conjugated EGFR antibody (green) with DAPI nuclear stain (blue).
IHC testing of lung squamous cell carcinoma stained with EGFR antibody (ERB59-1).
FACS testing of A431 cells with isotype control (gray) and EGFR antibody (green).
Immunofluorescent testing of A431 cells and Alexa Fluor conjugated EGFR antibody (green) with DAPI nuclear stain (blue).

EGFR Antibody [ERB59-1]

CATALOG NUMBER: 33-893

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant human protein was used as the immunogen for this EGFR antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC
Application Note:
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EGFR antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2a, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
EGF R
Additional Names:
Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1
Ncbi Gene Id Number:
1956
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. [UniProt]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-893

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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