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IHC testing of squamous cell carcinoma stained with EGFR antibody (GFR450).
FACS testing of A431 cells with isotype control (gray) and EGFR antibody (green).
Immunofluorescent staining of A431 cells with Alexa Fluor conjugated EGFR antibody (green) and DAPI (nuclear, blue).
SDS-PAGE Analysis of Purified, BSA-Free EGFR Antibody (clone GFR450). Confirmation of Integrity and Purity of the Antibody.
IHC testing of squamous cell carcinoma stained with EGFR antibody (GFR450).

EGFR Antibody [GFR450]

CATALOG NUMBER: 33-119

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
The immunizing protein for this antibody was recombinant human EGFR.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC, WB
Application Note:
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
IF: 1-2 ug/ml
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EGFR antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2a, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
EGF R
Additional Names:
Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1
Ncbi Gene Id Number:
1956
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:

This antibody recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to ErbB2, -3 -4 or HER2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head/neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-119

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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