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<b> Flow-cytometry using the anti-EGFR antibody Matuzum34) </b> HeLa (A) and A431 (B) cells were stained with unimmunized r antibody (black line) or the rmeric version of Matuzum6- blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using an anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.
<b> Immunofluorescence staining of fixed A431 cells with anti-EGFR antibody Matuzum34) </b> Immunofluorescence analysis of unpermearaformaldehyde fixed A431 cells on Shi-fix™ coverslips stained with the chimeric rsion of Matuzum6) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-426- DAPI- merged channels and an isotype control. The isotype control was stained with unimmunised r followed by Alexa Fluor® 488 secondary antibody.
Flow-cytometry using the anti-EGFR antibody Matuzum34) HeLa (A) and A431 (B) cells were stained with unimmunized r antibody (black line) or the rmeric version of Matuzum6- blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using an anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.

EGFR Antibody [Matuzumab], Rabbit IgG – Research Grade Biosimilar

CATALOG NUMBER: 24-426

Clonality:
Recombinant Monoclonal
Tested Applications:
Antagonist, Blocking, ELISA, Flow, IF, WB
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Species Reactivity:
Human
Species:
Rabbit
Original Species Name:
Human
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Immunogen:
The parental mouse antibody was generated by immunizing BALB/c mice intraperitoneally with A431 cells in phosphate buffered saline (PBS). Later on the humanzied version of the antibody was created by grafting CDRs of the murine antibody onto human constant regions.
Conjugate:
Unconjugated
Tested Applications:
Antagonist, Blocking, ELISA, Flow, IF, WB
Clonality:
Recombinant Monoclonal
Isotype:
IgG kappa
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Storage Conditions:
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Ncbi Official Symbol:
C3
Additional Names:
ErbB-1, ERBB1, HER1, EGF, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EMD72000, mAb425, 425, mAb 425
Ncbi Gene Id Number:
718
User Note:
For research use only.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-426

List Size:
0.2 mg

List Price:

$598.00

Datasheet
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