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Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Antibody used in WB on Human HepG2 at 1.25 ug/ml.

EGLN1 Antibody

CATALOG NUMBER: 28-141

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EGLN1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
EGLN1 antibody can be used for detection of EGLN1 by ELISA at 1:12500. EGLN1 antibody can be used for detection of EGLN1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
46 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store EGLN1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
EGLN1
Additional Names:
EGLN1, HPH2, PHD2, SM20, ECYT3, HPH-2, HIFPH2, ZMYND6, C1orf12, HIF-PH2
Protein Accession Number:
NP_071334
Protein Gi Number:
13489073
Ncbi Gene Id Number:
54583
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
EGLN1 has a role in the regulation of hypoxia-inducible factor. It is not affected by overexpression or downregulation of HIF-2alpha.
Background Reference 1:
Appelhoff, R.J., et al., (2004) J. Biol. Chem. 279 (37), 38458-38465.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-141

List Size:
100 ul

List Price:

$473.00

Datasheet
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