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Western blot analysis in 293 cell line lysates (35ug/lane).
Western blot analysis in 293 cell line lysates (35ug/lane).

EGLN1 Antibody

CATALOG NUMBER: 60-009

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This EGLN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-425 amino acids from the C-terminal region of human EGLN1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
46 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EGLN1
Additional Names:
Egl nine homolog 1, Hypoxia-inducible factor prolyl hydroxylase 2, HIF-PH2, HIF-prolyl hydroxylase 2, HPH-2, Prolyl hydroxylase domain-containing protein 2, PHD2, SM-20, EGLN1 (HGNC:1232), C1orf12
Protein Accession Number:
Q9GZT9
Protein Gi Number:
32129514
Ncbi Gene Id Number:
54583
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene catalyzes thepost-translational formation of 4-hydroxyproline inhypoxia-inducible factor (HIF) alpha proteins. HIF is atranscriptional complex that plays a central role in mammalianoxygen homeostasis. This protein functions as a cellular oxygensensor, and under normal oxygen concentration, modification byprolyl hydroxylation is a key regulatory event that targets HIFsubunits for proteasomal destruction via the von Hippel-Lindauubiquitylation complex. Mutations in this gene are associated witherythrocytosis familial type 3 (ECYT3).
Background Reference 1:
Aggarwal, S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(44):18961-18966(2010)
Background Reference 2:
Vogel, S., et al. J. Biol. Chem. 285(44):33756-33763(2010)
Background Reference 3:
Simonson, T.S., et al. Science 329(5987):72-75(2010)
Background Reference 4:
Thoms, B.L., et al. J. Biol. Chem. 285(27):20472-20480(2010)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-009

List Size:
400 ul

List Price:

$495.00

Datasheet
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