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Antibody used in WB on Human Heart at 0.2-1 ug/ml.
Antibody used in WB on Human Heart at 0.2-1 ug/ml.

EGLN2 Antibody

CATALOG NUMBER: 25-225

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EGLN2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
EGLN2 antibody can be used for detection of EGLN2 by ELISA at 1:62500. EGLN2 antibody can be used for detection of EGLN2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10407 - Fetal Heart Tissue Lysate
Predicted Molecular Weight:
16 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store EGLN2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
EGLN2
Additional Names:
EGLN2, EIT6, PHD1, HPH-1, HPH-3, HIFPH1, HIF-PH1
Protein Accession Number:
EAW57007
Protein Gi Number:
119577411
Ncbi Gene Id Number:
112398
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The hypoxia inducible factor (HIF) is a transcriptional complex which is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. EGLN2 encodes an enzyme responsible for this posttranslational modification. Alternative splicing of EGLN2 results in three transcript variants encoding different isoforms.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

25-225

List Size:
100 ul

List Price:

$519.00

Datasheet
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