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Western blot analysis of extracts of various cell lines, using EGLN2 antibody (14-021).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse testis using EGLN2 antibody (14-021) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using EGLN2 antibody (14-021) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using EGLN2 antibody (14-021) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse prostate using EGLN2 antibody (14-021) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of SKOV-3 cells using EGLN2 antibody (14-021).
Immunofluorescence analysis of HeLa cells using EGLN2 antibody (14-021).
Immunofluorescence analysis of A549 cells using EGLN2 antibody (14-021).
Western blot analysis of extracts of various cell lines, using EGLN2 antibody (14-021).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

EGLN2 Antibody [A5D3]

CATALOG NUMBER: 14-021

Clonality:
Monoclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of human EGLN2
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
PC-12
Positive Control 3:
NIH/3T3
Predicted Molecular Weight:
Observed: 44kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
EGLN2
Additional Names:
EGLN2, EIT6, PHD1, HPH-1, HPH-3, HIFPH1, HIF-PH1
Ncbi Gene Id Number:
112398
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-021

List Size:
50 uL

List Price:

$335.00

Out of stock

Datasheet
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