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Antibody used in WB on Human Brain at 0.2-1 ug/ml.
Antibody used in WB on Human Brain at 0.2-1 ug/ml.

EGLN3 Antibody

CATALOG NUMBER: 30-813

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EGLN3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
EGLN3 antibody can be used for detection of EGLN3 by ELISA at 1:1562500. EGLN3 antibody can be used for detection of EGLN3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10123 - Fetal Brain Tissue Lysate
Predicted Molecular Weight:
27 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store EGLN3 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
EGLN3
Additional Names:
EGLN3, FLJ21620, HIFPH3, MGC125998, MGC125999, PHD3, HIFP4H3
Protein Accession Number:
NP_071356
Protein Gi Number:
11545787
Ncbi Gene Id Number:
112399
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
EGLN3 catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-564', and HIF-2 alpha. EGLN3 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. It may play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. EGLN3 promotes cell death through a caspase-dependent mechanism.
Background Reference 1:
Ginouves, A., (2008) Proc. Natl. Acad. Sci. U.S.A. 105 (12), 4745-4750.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-813

List Size:
100 ul

List Price:

$519.00

Datasheet
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