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Western blot analysis in HL-60 cell line lysates (35ug/lane).
EGLN3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in HL-60 cell line lysates (35ug/lane).

EGLN3 Antibody

CATALOG NUMBER: 57-569

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This EGLN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 203-231 amino acids from the C-terminal region of human EGLN3.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
27 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EGLN3
Additional Names:
Egl nine homolog 3, HPH-1, Hypoxia-inducible factor prolyl hydroxylase 3, HIF-PH3, HIF-prolyl hydroxylase 3, HPH-3, Prolyl hydroxylase domain-containing protein 3, PHD3, EGLN3
Protein Accession Number:
Q9H6Z9
Protein Gi Number:
32129515
Ncbi Gene Id Number:
112399
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
EGLN3 catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. Promotes cell death through a caspase-dependent mechanism (By similarity).
Background Reference 1:
Sato, M., et al. Exp. Cell Res. 316(17):2871-2882(2010)
Background Reference 2:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 3:
Xue, J., et al. Gastroenterology 138(2):606-615(2010)
Background Reference 4:
Henze, A.T., et al. Cancer Res. 70(1):357-366(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-569

List Size:
400 ul

List Price:

$495.00

Datasheet
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