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Antibody used in WB on Mouse NIH-3T3 at 2.5 ug/ml.
Antibody used in IHC on Xenopus Laevis at: 1:500.
Antibody used in IHC on Xenopus Laevis at 1:500.
Antibody used in WB on Mouse NIH-3T3 at 2.5 ug/ml.

EGR1 Antibody

CATALOG NUMBER: 28-572

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse EGR1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
EGR1 antibody can be used for detection of EGR1 by ELISA at 1:12500. EGR1 antibody can be used for detection of EGR1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Predicted Molecular Weight:
59 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store EGR1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
EGR1
Additional Names:
EGR1, egr, TIS8, Zenk, Egr-1, NGFIA, Zfp-6, ETR103, Krox-1, Krox24, NGF1-A, NGFI-A, Zif268, Krox-24, A530045N19Rik
Protein Accession Number:
NP_031939
Protein Gi Number:
6681285
Ncbi Gene Id Number:
13653
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Early Growth Response Protein 1 (EGR1, Krox-24 protein, nerve growth factor-induced protein A, Transcription factor ETR103, Zinc finger protein 225) belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene.
Background Reference 1:
Schnell, F.J., et al., (2006) J. Immunol. 176 (8), 4740-4747.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-572

List Size:
100 ul

List Price:

$473.00

Datasheet
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