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Antibody used in WB on Human Jurkat 0.0625 ug/ml.
Antibody used in WB on Human Jurkat 0.0625 ug/ml.

EGR4 Antibody

CATALOG NUMBER: 28-787

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EGR4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
EGR4 antibody can be used for detection of EGR4 by ELISA at 1:312500. EGR4 antibody can be used for detection of EGR4 by western blot at 0.0625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
51 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store EGR4 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
EGR4
Additional Names:
EGR4, NGFIC, NGFI-C, PAT133
Protein Accession Number:
NP_001956
Protein Gi Number:
237512940
Ncbi Gene Id Number:
1961
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The nerve growth factor-induced clone C (NGFI-C/EGR4) gene is a zinc-finger transcription factor that is rapidly induced by nerve growth factor in rat pheochromocytoma PC12 cells and by seizure in brain. NGFI-C/EGR4 is closely related to the previously described early response genes, nerve growth factor-induced clone A (NGFI-A or EGR1), EGR2, and EGR3. These four early response (immediate early) proteins all contain very similar zinc-finger DNA binding domains and five highly homologous subdomains. EGR-4 functionally cooperate with NFAT proteins and induce expression of IL-2 and TNFalpha. Early growth response proteins (EGR) and nuclear factors of activated T cells (NFAT) form heterodimers and regulate proinflammatory cytokine gene expression.
Background Reference 1:
Decker, E.L., (2003) Nucleic Acids Res. 31 (3), 911-921.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-787

List Size:
100 ul

List Price:

$519.00

Datasheet
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