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Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.

EHD1 Antibody

CATALOG NUMBER: 57-288

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This EHD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 410-438 amino acids from the C-terminal region of human EHD1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
61 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EHD1
Additional Names:
EH domain-containing protein 1, PAST homolog 1, hPAST1, Testilin, EHD1, PAST, PAST1
Protein Accession Number:
Q9H4M9
Protein Gi Number:
18202945
Ncbi Gene Id Number:
10938
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene belongs to a highly conserved gene familyencoding EPS15 homology (EH) domain-containing proteins. Theprotein-binding EH domain was first noted in EPS15, a substrate forthe epidermal growth factor receptor. The EH domain has been shownto be an important motif in proteins involved in protein-proteininteractions and in intracellular sorting. The protein encoded bythis gene is thought to play a role in the endocytosis of IGF1receptors.
Background Reference 1:
Gudmundsson, H., et al. Circ. Res. 107(1):84-95(2010)
Background Reference 2:
Sharma, M., et al. Mol. Biol. Cell 20(24):5181-5194(2009)
Background Reference 3:
Kieken, F., et al. Protein Sci. 18(12):2471-2479(2009)
Background Reference 4:
Jovic, M., et al. Mol. Biol. Cell 20(11):2731-2743(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-288

List Size:
400 ul

List Price:

$495.00

Datasheet
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