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45-523 (0.1µg/ml) staining of A549 (A) and (0.3µg/ml) HeLa (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
45-523 (0.3µg/ml) staining of Human Lung lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
45-523 (0.1µg/ml) staining of A549 (A) and (0.3µg/ml) HeLa (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

EHD2 Antibody

CATALOG NUMBER: 45-523

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: CRLVPPSKRRHKGSA
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 65kDa band observed in Human Placenta lysates (calculated MW of 61.2kDa according to NP_055416.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunofluorescence: strong expression of the protein seen in the plasma membrane of A431 cells and the nuclei of HeLa cells. Recommended concentration: 10µg/ml.Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
Specificity:
This antibody is expected to recognise EHD1 protein as well as EHD2.
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
EHD2
Additional Names:
EHD2, PAST2, EH-domain containing 2, EH domain containing 2, FLJ96617
Protein Accession Number:
NP_055416.2
Protein Gi Number:
21361462
Ncbi Gene Id Number:
30846
Background Reference 1:
Guilherme A, Soriano NA, Furcinitti PS, Czech MP. Role of EHD1 and EHBP1 in perinuclear sorting and insulin-regulated GLUT4 recycling in 3T3-L1 adipocytes. J Biol Chem. 2004 Sep 17;279(38):40062-75. Epub 2004 Jul 09.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-523

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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