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Western blot analysis of extracts of various cell lines, using EHHADH antibody (15-015) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using EHHADH antibody (15-015) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using EHHADH antibody (15-015) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using EHHADH antibody (15-015). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using EHHADH antibody (15-015) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

EHHADH Antibody

CATALOG NUMBER: 15-015

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 444-723 of human EHHADH (NP_001957.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
HepG2
Positive Control 2:
Mouse liver
Positive Control 3:
Rat liver
Positive Control 4:
Rat kidney
Predicted Molecular Weight:
Observed: 79kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
EHHADH
Additional Names:
Peroxisomal bifunctional enzyme, PBE, PBFE, Enoyl-CoA hydratase/3,2-trans-enoyl-CoA isomerase, 3-hydroxyacyl-CoA dehydrogenase, EHHADH, ECHD
Ncbi Gene Id Number:
1962
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomal disorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-015

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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