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Western blot analysis in Jurkat cell line lysates (15ug/lane).
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in Jurkat cell line lysates (15ug/lane).

EHMT2 Antibody

CATALOG NUMBER: 61-962

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This EHMT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-186 amino acids from the N-terminal region of human EHMT2.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
132 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EHMT2
Additional Names:
Histone-lysine N-methyltransferase EHMT2, 211-, Euchromatic histone-lysine N-methyltransferase 2, HLA-B-associated transcript 8, Histone H3-K9 methyltransferase 3, H3-K9-HMTase 3, Lysine N-methyltransferase 1C, Protein G9a, EHMT2, BAT8, C6orf30, G9A, KMT1C, NG36
Protein Accession Number:
Q96KQ7
Protein Gi Number:
116241348
Ncbi Gene Id Number:
10919
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
A cluster of genes, BAT1-BAT5, has been localized in thevicinity of the genes for TNF alpha and TNF beta. This gene isfound near this cluster; it was mapped near the gene for C2 withina 120-kb region that included a HSP70 gene pair. These genes areall within the human major histocompatibility complex class IIIregion. This gene was thought to be two different genes, NG36 andG9a, adjacent to each other but a recent publication shows thatthere is only a single gene. The protein encoded by this gene isthought to be involved in intracellular protein-proteininteraction.
Background Reference 1:
Goyama, S., et al. Leukemia 24(1):81-88(2010)
Background Reference 2:
de Vogel, S., et al. Cancer Epidemiol. Biomarkers Prev. 18(11):3086-3096(2009)
Background Reference 3:
Chen, X., et al. J. Biol. Chem. 284(41):27857-27865(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-962

List Size:
400 ul

List Price:

$495.00

Datasheet
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