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Western blot analysis of EI24 in rat liver tissue lysate with EI24 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of EI24 in rat liver tissue with EI24 antibody at 5 μg/mL.
Western blot analysis of EI24 in rat liver tissue lysate with EI24 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

EI24 Antibody

CATALOG NUMBER: 6487

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (95%)
Immunogen:
EI24 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human EI24.
The immunogen is located within the last 50 amino acids of EI24.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
EI24 antibody can be used for detection of EI24 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in rat samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of EI24 are known to exist; this antibody will only detect the larger isoform.
Positive Control 1:
Cat. No. 1464 - Rat Liver Tissue Lysate
Purification:
EI24 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
EI24 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
EI24 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EI24
Additional Names:
EI24 Antibody: EPG4, PIG8, TP53I8, p53-induced gene 8 protein
Protein Accession Number:
NP_004870
Protein Gi Number:
55956766
Ncbi Gene Id Number:
9538
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
EI24 Antibody: The etoposide-induced 24 (EI24) protein is an immediate-early induction target of p53-mediated apoptosis and functions as a tumor suppressor. EI24 contains six transmembrane domains and is localized to the endoplasmic reticulum (ER). It binds to Bcl-2 and may contribute to apoptosis by modulating the activity and function of Bcl-2. EI24 is highly mutated in aggressive breast cancers and its loss in breast cancer tissue correlates with tumor invasiveness. Similar findings in invasive cervical carcinoma suggest that EI24 may be a promising therapeutic target in the treatment of invasive cancer.
Background Reference 1:
Lehar SM, Nacht M, Jacks T, et al. Identification and cloning of EI24, a gene induced by p53 in etoposide-treated cells. Oncogene 1996; 12:1181-7.
Background Reference 2:
Gu Z, Flemington C, Chittenden T, et al. ei24, a p53 response gene involved in growth suppression and apoptosis. Mol. Cell Biol. 2000; 20:233-41
Background Reference 3:
Zhao X, Ayer RE, Davis SL, et al. Apoptosis factor EI24/PIG8 is a novel endoplasmic reticulum-localized Bcl-2-binding protein which is associated with suppression of breast cancer invasiveness. Cancer Res. 2005; 65:2125-9.
Background Reference 4:
Mazumder D, Mitra S, Singh RK, et al. Inactivation of CHEK1 and EI24 are associated with the development of invasive cervical carcinoma: clinical and prognostic implications. Int. J. Cancer 2010; epub

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6487

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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