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Western blot analysis of extracts of various cell lines, using EIF1AX antibody (19-941) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human stomach using EIF1AX antibody (19-941) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EIF1AX antibody (19-941) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using EIF1AX Polyclonal Antibody (19-941) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using EIF1AX Polyclonal Antibody (19-941) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using EIF1AX antibody (19-941) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

EIF1AX Antibody

CATALOG NUMBER: 19-941

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 10-144 of human EIF1AX (NP_001403.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:200 - 1:2000
IHC: 1:100 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
SKOV3
Positive Control 2:
HT-29
Positive Control 3:
MCF7
Positive Control 4:
NCI-H460
Positive Control 5:
Mouse brain
Positive Control 6:
Mouse kidney
Predicted Molecular Weight:
Observed: 16kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
EIF1AX
Additional Names:
EIF1A, EIF1AP1, EIF4C, eIF-1A, eIF-4C, eukaryotic translation initiation factor 1A, X-chromosomal, Putative eukaryotic translation initiation factor 1A, eIF-1A X isoform, eukaryotic translation initiation factor 1A, X chromosome, eukaryotic translation initiation factor 4C
Ncbi Gene Id Number:
1964
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes an essential eukaryotic translation initiation factor. The protein is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-941

List Size:
100 uL

List Price:

$436.00

Datasheet
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