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Western blot analysis of EIF2A Antibody in Ramos cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with EIF2A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of EIF2A Antibody in Ramos cell line lysates (35ug/lane)

EIF2A Antibody

CATALOG NUMBER: 63-985

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This EIF2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 437-465 amino acids from the Central region of human EIF2A.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
65 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EIF2A
Additional Names:
Eukaryotic translation initiation factor 2A, eIF-2A, 65 kDa eukaryotic translation initiation factor 2A, Eukaryotic translation initiation factor 2A, N-terminally processed, EIF2A
Protein Accession Number:
Q9BY44
Protein Gi Number:
209572747
Ncbi Gene Id Number:
83939
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes (Zoll et al., 2002 [PubMed 12133843]).
Background Reference 1:
Willis, K.L., et al. Virology 394(1):73-81(2009)
Background Reference 2:
Spurgeon, M.E., et al. J. Virol. 83(19):9970-9982(2009)
Background Reference 3:
Lu, W., et al. J. Biol. Chem. 284(36):24281-24288(2009)
Background Reference 4:
Yang, J., et al. Mol. Cell. Biol. 29(8):2243-2253(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-985

List Size:
400 ul

List Price:

$495.00

Datasheet
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