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Western blot analysis of lysed extracts from Jurkat cells untreated or treated with H2O2 using eIF2α (Phospho-Ser51).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2α (Phospho-Ser51).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using eIF2α(Phospho-Ser51).
Western blot analysis of lysed extracts from Jurkat cells untreated or treated with H2O2 using eIF2α (Phospho-Ser51).

eIF2a (phospho Ser51) Antibody

CATALOG NUMBER: 79-226

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
eIF2a (Phospho-Ser51) antibody was raised against a peptide sequence around phosphorylation site of serine 51 (E-L-S (p) -R-R) derived from Human eIF2α.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of eIF2α only when phosphorylated at serine 51.
Predicted Molecular Weight:
38 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
EIF2S1
Additional Names:
EIF2, EIF-2, EIF2A, EIF-2A, EIF-2alpha, STK14A, Eukaryotic translation initiation factor 2 subunit alpha
Protein Accession Number:
NP_004085.1
Protein Gi Number:
4758256
Ncbi Gene Id Number:
1965
Background:
Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Background Reference 1:
Xavier Saelens, et al. (2001) J. Biol. Chem; 276: 41620 - 41628. Hiroyuki Kubota, et al. (2003) J. Biol. Chem ; 278: 20457 - 20460. Shijian Chu, et al. (2006) Am J Physiol Lung Cell Mol Physiol ; 291: L983 - L992. Eileen Connolly, et al. (2006) Mol. Cell. Biol ; 26: 3955 - 3965.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-226

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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