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Antibody used in WB on Human Lung at 0.2-1 ug/ml.
Antibody used in WB on Human Hela at 1 ug/ml.
Antibody used in WB on Human HEK293T cells at 1 ug/ml.
Antibody used in WB on Human Lung at 0.2-1 ug/ml.

EIF2AK1 Antibody

CATALOG NUMBER: 25-766

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF2AK1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
EIF2AK1 antibody can be used for detection of EIF2AK1 by ELISA at 1:62500. EIF2AK1 antibody can be used for detection of EIF2AK1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10410 - Fetal Lung Tissue Lysate
Predicted Molecular Weight:
71 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store EIF2AK1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
EIF2AK1
Additional Names:
EIF2AK1, HRI, KIAA1369, HCR
Protein Accession Number:
NP_055228
Protein Gi Number:
11125768
Ncbi Gene Id Number:
27102
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
EIF2AK1 acts at the level of translation initiation to downregulate protein synthesis in response to stress. The protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene.The HRI gene is localized to 7p22 where its 3' end slightly overlaps the 3' end of the gene JTV1. The two genes are transcribed from opposite strands. Studies in rat and rabbit suggest that the HRI gene product phosphorylates the alpha subunit of eukaryotic initiation factor 2. Its kinase activity is induced by low levels of heme and inhibited by the presence of heme. Sequence Note: The sequence AF181071.1 is a chimeric mRNA clone. Only the heme-regulated initiation factor 2-alpha kinase region was propagated into this RefSeq record.
Background Reference 1:
Olsen, J.V., (2006) Cell 127 (3), 635-648.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

25-766

List Size:
100 ul

List Price:

$519.00

Datasheet
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