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Western blot analysis of extracts of HepG2 cells, using EIF2AK4 Antibody (14-545) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat testis using EIF2AK4 antibody (14-545) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using EIF2AK4 antibody (14-545) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using EIF2AK4 antibody (14-545) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using EIF2AK4 antibody (14-545) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EIF2AK4 antibody (14-545) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using EIF2AK4 antibody (14-545). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of HepG2 cells, using EIF2AK4 Antibody (14-545) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

EIF2AK4 Antibody

CATALOG NUMBER: 14-545

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human EIF2AK4 (NP_001013725.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
HepG2
Predicted Molecular Weight:
Observed: 187kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
EIF2AK4
Additional Names:
EIF2AK4, GCN2, GCN2 eIF2alpha kinase, GCN2-like protein, Mgcn2, KIAA1338
Ncbi Gene Id Number:
440275
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-545

List Size:
100 uL

List Price:

$436.00

Datasheet
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