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Western blot analysis in 293 cell line lysates (35ug/lane).
EIF2B1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in 293 cell line lysates (35ug/lane).

EIF2B1 Antibody

CATALOG NUMBER: 56-411

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This EIF2B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 136-163 amino acids from the Central region of human EIF2B1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
34 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EIF2B1
Additional Names:
Translation initiation factor eIF-2B subunit alpha, eIF-2B GDP-GTP exchange factor subunit alpha, EIF2B1, EIF2BA
Protein Accession Number:
Q14232
Protein Gi Number:
2494303
Ncbi Gene Id Number:
1967
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes one of five subunits of eukaryotictranslation initiation factor 2B (EIF2B), a GTP exchange factor foreukaryotic initiation factor 2 and an essential regulator forprotein synthesis. Mutations in this gene and the genes encodingother EIF2B subunits have been associated with leukoencephalopathywith vanishing white matter.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Background Reference 3:
Hiyama, T.B., et al. J. Mol. Biol. 392(4):937-951(2009)
Background Reference 4:
Pronk, J., et al. Mult. Scler. 14(8):1123-1126(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-411

List Size:
400 ul

List Price:

$495.00

Datasheet
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