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Western Blot at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.

EIF2B3 Antibody

CATALOG NUMBER: 58-888

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This EIF2B3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 413-441 amino acids from the C-terminal region of human EIF2B3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
50 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EIF2B3
Additional Names:
Translation initiation factor eIF-2B subunit gamma, eIF-2B GDP-GTP exchange factor subunit gamma, EIF2B3
Protein Accession Number:
Q9NR50
Protein Gi Number:
18203317
Ncbi Gene Id Number:
8891
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is one of the subunits ofinitiation factor eIF2B, which catalyzes the exchange of eukaryoticinitiation factor 2-bound GDP for GTP. It has also been found tofunction as a cofactor of hepatitis C virus internal ribosome entrysite-mediated translation. Mutations in this gene have beenassociated with leukodystrophy with vanishing white matter.Alternatively spliced transcript variants encoding differentisoforms have been found for this gene.
Background Reference 1:
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Background Reference 2:
Pronk, J., et al. Mult. Scler. 14(8):1123-1126(2008)
Background Reference 3:
Maletkovic, J., et al. J. Child Neurol. 23(2):205-215(2008)
Background Reference 4:
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-888

List Size:
400 ul

List Price:

$495.00

Datasheet
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