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42-814 (0.3ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-814 (0.3ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

EIF2B4 Antibody

CATALOG NUMBER: 42-814

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-RKDYGSKVSLFSH
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 60kDa band observed in lysates of cell line K562 (calculated MW of 59.6kDa according to NP_751945.2). Recommended concentration: 0.3-1ug/ml.An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificity:
This antibody is expected to recognize all reported isoforms (NP_751945.2; NP_001029288.1; NP_056451.3).
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
EIF2B4
Additional Names:
EIF2B4, eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa, DKFZp586J0119, EIF-2B, EIF2B, EIF2Bdelta, eIF-2B GDP-GTP exchange factor subunit delta, eukaryotic translation initiation factor 2B, subunit 4 delta, translation initiation factor eIF-2b delta subunit
Protein Accession Number:
NP_751945.2,NP_001029288.1,NP_056451.3
Protein Gi Number:
187607323
Ncbi Gene Id Number:
8890

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-814

List Size:
0.1 mg

List Price:

$469.00

Out of stock

Datasheet
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