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Western blot analysis of extracts of various cell lines, using AGO2 antibody (19-980) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human stomach using AGO2 antibody (19-980) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using AGO2 antibody (19-980) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using AGO2 antibody (19-980) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using AGO2 antibody (19-980) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

EIF2C2 Antibody

CATALOG NUMBER: 19-980

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EIF2C2 (NP_036286.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:1000
IHC: 1:50 - 1:200
Positive Control 1:
Mouse heart
Positive Control 2:
Mouse brain
Positive Control 3:
Rat brain
Positive Control 4:
Rat heart
Predicted Molecular Weight:
Observed: 97kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
AGO2
Additional Names:
CASC7, EIF2C2, LINC0098PPD, Q1protein argonaute-2, PAZ Piwi domain protein, argonaute 2, RISC catalytic component, cancer susceptibility candidate 7, cancer susceptibility candidate 7 (non-protein coding), eukaryotic translation initiation factor 2C, 2, long intergenic non-protein coding RNA 98protein slicer
Ncbi Gene Id Number:
27161
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-980

List Size:
100 uL

List Price:

$436.00

Datasheet
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