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Western blot analysis in mouse bladder tissue lysates (35ug/lane).
Confocal immunofluorescent analysis of EIF2S1 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
EIF2S1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in mouse bladder tissue lysates (35ug/lane).

EIF2S1 Antibody

CATALOG NUMBER: 57-063

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat, Chicken, Yeast, Bovine, Drosophila
Immunogen:
This EIF2S1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-89 amino acids from the N-terminal region of human EIF2S1.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
36 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EIF2S1
Additional Names:
Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, EIF2S1, EIF2A
Protein Accession Number:
P05198
Protein Gi Number:
124200
Ncbi Gene Id Number:
1965
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The translation initiation factor EIF2 catalyzes the firstregulated step of protein synthesis initiation, promoting thebinding of the initiator tRNA to 40S ribosomal subunits. Bindingoccurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2is composed of 3 nonidentical subunits, the 36-kD EIF2-alphasubunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908),and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate offormation of the ternary complex is modulated by thephosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed2948954]).
Background Reference 1:
Shiota, M., et al. Int. J. Oncol. 36(6):1521-1531(2010)
Background Reference 2:
Kulkarni, A.P., et al. Indian J. Biochem. Biophys. 47(2):67-74(2010)
Background Reference 3:
Wehner, K.A., et al. Mol. Cell. Biol. 30(8):2006-2016(2010)
Background Reference 4:
Backes, S., et al. J. Gen. Virol. 91 (PT 2), 470-482 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-063

List Size:
400 ul

List Price:

$495.00

Datasheet
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