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Antibody used in WB on Human Jurkat 5.0 ug/ml.
Antibody used in WB on Human Jurkat 5.0 ug/ml.

EIF2S3 Antibody

CATALOG NUMBER: 30-144

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat, Zebrafish
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat, Zebrafish
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF2S3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
EIF2S3 antibody can be used for detection of EIF2S3 by ELISA at 1:312500. EIF2S3 antibody can be used for detection of EIF2S3 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
51 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store EIF2S3 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
EIF2S3
Additional Names:
EIF2S3, EIF2, EIF2G, EIF2gamma, eIF-2gA
Protein Accession Number:
NP_001406
Protein Gi Number:
4503507
Ncbi Gene Id Number:
1968
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Background Reference 1:
Gevaert, K., (2003) Nat. Biotechnol. 21 (5), 566-569.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-144

List Size:
100 ul

List Price:

$473.00

Datasheet
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