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Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Antibody used in WB on Human, Mouse at 1:1000.
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.

EIF3E Antibody

CATALOG NUMBER: 26-291

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF3E.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
EIF3E antibody can be used for detection of EIF3E by ELISA at 1:62500. EIF3E antibody can be used for detection of EIF3E by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
52 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store EIF3E antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
EIF3E
Additional Names:
EIF3E, EIF3-P48, EIF3S6, INT6, eIF3-p46
Protein Accession Number:
NP_001559
Protein Gi Number:
4503521
Ncbi Gene Id Number:
3646
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
EIF3E belongs to the eIF-3 subunit E family.It is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. EIF3E is required for nonsense-mediated mRNA decay (NMD); It may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. The protein may interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.
Background Reference 1:
Buchsbaum, S., (2007) Oncogene 26 (35), 5132-5144.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

26-291

List Size:
100 ul

List Price:

$519.00

Datasheet
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